Table 1.
Kinetic Parameters for Wild-Type STR1 and Its Mutants
|
Km (μM)
|
|||
|---|---|---|---|
| STR1 | kcat (min−1) | Tryptamine | Secologanin |
| Wild type | 78.200 | 6.2 | 39 |
| Y151F | 57.700 | 17.2 | 44 |
| H307A | 1.800 | 7.9 | 5070 |
| E309A | 0.089 | 5.4 | 95 |
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