FIG. 3.

(Top, left) Representative titration experiment showing the sensitization activities of some bis-acyl analogues. E. coli ATCC 9637 was seeded in MHB in checkerboard format in a 384-well plate containing a constant concentration of compound and varying doses of rifampin. Bacterial growth was monitored by turbidimetry at 600 nm. PMBN and melittin were used as positive controls. Wells containing no test compound served as negative controls. (Top, right) Table of values showing sensitization to Rifampin by all compound 4 and 8 series compounds. (Bottom) Plot of OM and IM permeabilization activity against extent of sensitization by the acylpolyamines. Relative sensitization (n-fold) was calculated as the MIC of rifampin alone/MIC of rifampin plus 10 μM Compound.