FIG. 2.

FLG-TP is a competitive inhibitor of dGTP, the natural substrate of DHBV polymerase. Competitive inhibition of the incorporation of dGMP by FLG-TP was performed as described in the legend to Fig. 1A with [α-³²P]dGTP at a final concentration of 0.165 μM (⧫) or 0.825 μM (▪) with increasing concentrations of FLG-TP (0, 0.5, 1, 10, 50, and 100 μM). Viral nascent minus-strand DNA was analyzed quantitatively by a dot assay, and the results of the inhibition of [α-³²P]dGMP incorporation in viral DNA were plotted on the graph to determine the IC₅₀. The graph shown in this figure corresponds to the mean curve of inhibition of [α-³²P]dGMP incorporation according to three independent experiments. Error bars indicate standard deviation.