Skip to main content
. 2006 Mar;50(3):955–961. doi: 10.1128/AAC.50.3.955-961.2006

FIG. 3.

FIG. 3.

Activity of FLG on the replication of wild-type and drug-resistant mutants of HBV in transiently transfected Huh7 cells. The antiviral activity of FLG was analyzed after transient transfection of Huh7 cells with either wild-type HBV genome (wt) or with 3TC-resistant (3TC-R; rtL180M+M204V), PMEA-resistant (PMEA-R; rtN236T), or 3TC+PMEA-resistant (3TC+PMEA-R; rtL180M+M204V+N236T) HBV genome cloned in pTriEXMod-HBV (6). Sixty hours posttransfection, treatment with increasing concentrations of FLG (0, 1, 5, 10, 25, and 100 μM) was started. Media were changed daily, and drug administration was performed from day 3 to day 8 posttransfection. Cells were then lysed, and HBV DNA was purified from intracellular core particles, separated on agarose gels, and submitted to Southern blot analysis. M.W., molecular weight; Lin. HBV, linear HBV DNA.