Virulence of B. abortus 2308 pBBR4 (black squares), the pcs mutant (open triangles), and the complemented pcs pBBR-pcs strain in BALB/c mice. Mice were infected by intraperitoneal injection. Individual spleen CFU values were plotted, and the horizontal bars represent the median CFU for each treatment group. *, P < 0.05 (compared to the group that received the wild-type strain). Statistical analysis was performed with a Kruskal-Wallis test. p.i., postinfection.