FIG.5.

Treatment of R. sphaeroides populations with amdinocillin results in phenotypic effects on cell shape prior to cell lysis. (A) DIC image of a population of R. sphaeroides incubated with amdinocillin for 1 h. The black arrows highlight cells that displayed increased cell widths; the white arrows highlight cells with increased cell lengths. The scale bar represents 1 μm. (B) A population of R. sphaeroides without amdinocillin. (C) Comparison of the lengths and widths of cells treated with amdinocillin and untreated. □, untreated cells; ⧫, cells treated with amdinocillin. A total of 247 cells were analyzed. Amdinocillin treatment increased the mean cell width from 1.17 ± 0.04 μm to 1.46 ± 0.01 μm, while the mean cell length was increased from 2.39 ± 0.05 μm to 2.95 ± 0.07 μm. The errors in the cell length/width refer to the standard errors of the mean.