TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Name | Genotype or relevant characteristics | Source or reference |
|---|---|---|---|
| Strains | |||
| HAK006 | ΔkdpABCD Δ(lac-pro) ara thi kdpFABC promoter-lacZ+ fusion gene at the att λ site | 19 | |
| TKV2208 | ΔkdpD trkA405 trkD1 nagA thi rha lacZ | 20 | |
| MC1061 | kdpD+hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galU galK rpsL thi | 16 | |
| HMK006 | ΔkdpD Δ(lac-pro) ara thi kdpFABC promoter-lacZ+ fusion gene at the att λ site | This study | |
| DY330 | W3110 ΔlacU169 gal490 (λcI857Δcro-broA) | 28 | |
| Plasmids | |||
| pBD | KdpD | Apr, pBAD18, kdpD | 11 |
| pSF1 | KdpD-N | Cmr, pBAD33, kdpDΔ449-894 | This study |
| pSF2 | KdpD-C | Apr, pBAD18, kdpDΔ1-443 | This study |
| pMR1 | N1-2C | Apr, pBAD18, kdpDΔ446-496 | This study |
| pSF3 | N3-4C | Apr, pBAD18, kdpDΔ398-446 | This study |
| pSF4 | N1-4C | Apr, pBAD18, kdpDΔ423-476 | This study |
| pMR2 | H4+C | Apr, pBAD18, kdpDΔ1-466 | This study |
| pMR3 | H4+C+RR | Apr, pBAD18, kdpDΔ1-466, (D474R, V475R) derivative of pMR2 | This study |
| pMR4 | C494-894 | Apr, pBAD18, kdpDΔ1-493 | This study |
| pMR5 | C499-894 | Apr, pBAD18, kdpDΔ1-498 | This study |
| pKD3 | Apr, Cmr, PCR template | 4 | |
| pCP20 | Apr, Cmr, ts FLP recombinase | 3 |