TABLE 2.

Growth of KdpD constructs in E. coli TKV2208 under K⁺-limiting conditions

KdpD construct	Cell densitya (OD600)	Growth rateb (h−1)	Colony formationc
KdpD	0.89 ± 0.07	0.45	+
KdpD-N	0.06 ± 0.09	0.00	−
N1-2C	0.82 ± 0.11	0.18	+
KdpD-C	0.94 ± 0.12	0.45	+
N3-4C	0.89 ± 0.08	0.27	+
N1-4C	0.95 ± 0.07	0.33	+
H4+C	0.99 ± 0.06	0.39	+
H4+C+RR	0.93 ± 0.03	0.40	+
C494-894	0.88 ± 0.10	0.43	+
C499-894	0.95 ± 0.11	0.44	+

^aCells were grown overnight in minimal medium containing 0.1 mM KCl.

^bOvernight cultures were grown in KML complex medium (1% KCl, 1% tryptone, 0.5% yeast extract), washed in minimal medium without K⁺, and diluted 1:50 in minimal medium containing 0.1 mM KCl.

^cCells were streaked on minimal agar plates containing 0.1 mM KCl.