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Real-time quantitative RT-PCR analysis of plcH wild type and the plcHΔss mutant
| Time after IPTG induction (min) | Relative abundancea of:
|
|
|---|---|---|
| plcH wild-type signal sequence | plcHΔss mutant | |
| 0 | 362 | .007 |
| 1 | 371 | .012 |
| 7 | 881 | .013 |
| 15 | 1,028 | .031 |
| 30 | 1,256 | .036 |
Values are expressed as relative abundance of plcH wild-type or plcHΔss transcripts compared to that for the omlA transcripts. omlA is a constitutively expressed gene.
