TABLE 4.

PlcH NPPC activity in bacterial fractions^a of the PlcH Δsignal sequence mutant

P. aeruginosa strain	Activityb for:
	S	OM	P	IM	C	Total unitsc
Vector control	0.04	0.10	0.12	0.12	0.06	0.44
PlcH	7.24	0.54	0.80	2.08	0.16	10.82
PlcH Δss	0.04	0.08	0.07	0.08	0.14	0.40

^aAll cell fractions were adjusted based on total bacterial pellet weight for each sample obtained prior to fractionation. S, supernatant.

^bAssayed by hydrolysis of the synthetic substrate NPPC and expressed as activity units ΔOD₄₁₅/Δtime ×100.

^cTotal PlcH NPPC activity units for each construct represent the sum total activity of all the fractions.