TABLE 1.
Primers used in real-time RT-PCR with SYBR green probes
| Primer | N315 ORFa | Sequence |
|---|---|---|
| SG16S1 | SArRNA01 | CGTGCTACAATGGACAATACAAA |
| SG16S2 | SArRNA01 | ATCTACGATTACTAGCGATTCCA |
| SGhu1 | SA1305 | GCTGGGATATCAATTTCTTTACC |
| SGhu2 | SA1305 | ATCCAAAACTCACTTGCTAAAGG |
| SGsarA1 | SA0573 | CTGTATTGACATACATCAGCGAA |
| SGsarA2 | SA0573 | GTTCTTTCATCATGCTCATTACG |
| arcA3 | SA2428 | ATGTCAGGGGTACGTAAGGAA |
| arcA4 | SA2428 | GAGGCTTGTGGATCTCTAGTA |
| betB3 | SA2406 | ATCGCGAAGCGTTAGCACGA |
| betB4 | SA2406 | CGTCTTTATCTGCTAATCCAGC |
| lacA5 | SA1997 | TCAGATGAAGCTGGCAAACGA |
| lacA6 | SA1997 | GCTACAGCCAAAGTTGCATCA |
| marR3 | SA0904 | GAAGATTTGGACAACGCACGG |
| marR4 | SA0904 | GTTCCCTGCTAATCTTCATCG |
| lukD3 | SA1637 | ACTTAAGGCAGCCGGAAACAT |
| lukD4 | SA1637 | CATTTGATTCTGAACTAACCGAA |
| lytN3 | SA1090 | TGAAGGTGCTGTTAAAACATCG |
| lytN4 | SA1090 | CTTGGCTTGAGTTTTTCGGAGT |
| splA3 | SA1631 | CATTTGTGGGTGGTACTGGTG |
| splA4 | Sa1631 | CTCGAATGATGTGCTGATACTC |
| tcaR3 | SA2147 | GTAAATAAGGCCGCAGTAAGC |
| tcaR4 | SA2147 | GCTCTACCTTTGTCAGTTAAGG |
| wcaG3 | SA0123 | TGTATTTGGGCCAAGACAGGA |
| wcaG4 | SA0123 | CTAGTTTGCAGTCCGTCACC |
a
ORF, open reading frame.