TABLE 1.
Bacterial strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant characteristic(s) or sequence | Source, reference, or amplicon size |
|---|---|---|
| Strains | ||
| AG318 | DK1622 pNBC18::nla18 | 2 |
| AG339 | DK101 pNBC18::nla18 | This study |
| AG343 | DK1622 pNBC31::nla18 | 2 |
| AG390 | pNBC31::nla18 sdeK::Ω4408 Tn5lacZ | This study |
| AG391 | pNBC31::nla18 spi::Ω4521 Tn5lacZ | This study |
| AG392 | pNBC31::nla18 devR::Ω4414 Tn5lacZ | This study |
| AG393 | pNBC31::nla18 Ω4403 Tn5lacZ | This study |
| AG395 | pNBC31::nla18 Ω4435 Tn5lacZ | This study |
| AG407 | pNBC31::nla18 exo::Ω7536 Tn5lacZ | This study |
| AG1001 | AG343 Mx8attB::pFOR18 | This study |
| DK101 | pilQ1 (wild-type development) | 24 |
| DK476 | pilQ1 asgA476 | 19 |
| DK1622 | Wild-type development | 29 |
| DK4323 | pilQ1 asgA476 spi::Ω4521 Tn5lacZ | 39 |
| DK4300 | sdeK::Ω4408 Tn5lacZ | 37 |
| DK4368 | Ω4403 Tn5lacZ | 37 |
| DK4521 | spi::Ω4521 Tn5lacZ | 37 |
| DK5204 | Ω4435 Tn5lacZ | 37 |
| DK5208 | csgA::Tn5-132 ΩLS205 | 38 |
| DK5508 | devR::Ω4414 Tn5lacZ | 37 |
| DK7536 | exo::Ω7536 Tn5lacZ | 45 |
| DK11063 | fruA::Ω7540 Tn5lacZ | 64 |
| MS1000 | DK101ΔrelA1 | This study |
| Plasmids | ||
| pBGS18 | Kanr | 66 |
| pCR2.1-TOPO | Kanr | Invitrogen |
| pSWU19 | KanrattP | S. S. Wu and D. Kaiser |
| pSWU22 | Tetr | S. S. Wu and D. Kaiser |
| pBJ114 | Kanr, galK vector | 28 |
| pMS302 | 4.8 PstI fragment containing relA in pBGS18 | 23 |
| pMS302R | 4.8 PstI fragment containing relA (opposite orientation) in pBGS18 | This study |
| pMS330 | pMS302 with 1.3 kb of relA deleted | This study |
| pMS331 | pBJ114 carrying the 3.5-kb PstI fragment from pMS330 | This study |
| pNBC18 | 477-bp internal fragment of nla18 in pCR2.1-TOPO | 2 |
| pNBC31 | 477-bp internal fragment of nla18 in pSWU22 | 2 |
| pFOR18 | Kanr, pSWU19 containing 1.8 kb of nla18 on a BamHI-HindIII fragment | This study |
| Primers | ||
| relAfwd | 5′-TCATCGCCTTTCGCATCATCGC-3′ | 129 bp |
| relArev | 5′-ACATGTTGGGCTTCGGAATCGC-3′ |