TABLE 3.
The effects of metabolites on the activity of StoHK
| Metabolitea | Concentration (mM) | Remaining activity (%)b |
|---|---|---|
| ADP | 0.1 | 38 |
| 1 | 8.1 | |
| AMP | 0.1 | 76 |
| 1 | 27 | |
| CDP | 0.1 | 100 |
| 1 | 67 | |
| PPi | 1 | 100 |
| 10 | 46 | |
| Fructose-6-phosphate | 1 | 100 |
| 10 | 67 |
a
The following metabolites did not affect the enzyme activity: phosphoenolpyruvate, Pi, glyceraldehyde, citrate, lactate, pyruvate, fructose-1,6-bisphosphate, and G6P (10 mM each), and NAD+, NADH, NADP+, and NADPH (1 mM each).
b
The enzyme activity was measured at 50°C using half-saturating concentrations of glucose (0.050 mM) and ATP (0.12 mM) as substrate in the presence of 4 mM Mg2+.