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GlnJ accumulates in a glnB glnD background when GlnB* variants are expressed in single copy from an integrated plasmid. Cells were grown in MG medium, and proteins from crude extracts were separated by SDS-PAGE and immunoblotted with antibody against R. rubrum GlnJ. UR2 is the R. rubrum wild type (WT). UR1683 contains wild-type GlnB, while UR1685, -1687, -1689, -1691, -1693, and -1695 contain the GlnB* variants.
