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Purified CBD-Sec11a and CBD-Sec11b are active. CBD-Sec11a (A) and CBD-Sec11b (B) were purified as described in Materials and Methods and tested for their ability to release the SP domain from the reporter substrate SP-CBD. Incubations were conducted in the presence of purified enzyme and substrate, enzyme alone, or substrate alone, from 0 to 60 min. The collected aliquots were analyzed by SDS-PAGE and Coomassie blue stained.
