TABLE 1.
Bacterial strains and plasmids used for this study
| Strain or plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| E. coli strains | ||
| DH5α | φ80d lacZΔM15 endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 F− Δ(lacZYA-argF)U169 | 4 |
| S17-1 | thi pro hsdR recA Tra+ | 56 |
| KAM3 | ΔacrB Δ(lac-pro) supE thi hsdΔ5/F′ traD36 proA+B+lacIqlacZ ΔM15 | 36 |
| P. aeruginosa strains | ||
| K767 | PAO1, wild type | 29 |
| K1525 | K767 ΔmexXY | 58 |
| K2439 | K1525 ΔssrA | This study |
| K2440 | K1525 ΔsmpB | This study |
| K2413 | K767 ΔPA5471 | This study |
| K2414 | K767 ΔmexXY ΔPA5471 | This study |
| K2415 | K767 ΔmexZ | This study |
| K2416 | K767 ΔmexZ ΔPA5471 | This study |
| K2417 | K767 ΔPA5471-PA5470 | This study |
| K2437 | K767 ΔssrA | This study |
| K2438 | K767 ΔsmpB | This study |
| K2162 | Clinical isolate displaying MexXY-dependent pan-aminoglycoside resistance | 58 |
| K2418 | K2162 ΔPA5471 | This study |
| K2435 | K2162 PA5471::mini-Tn5-tet | This study |
| K2436 | K2162 PA5471::mini-Tn5-tet | This study |
| YM34 | PAO1 ΔmexAB ΔmexCD-oprJ ΔmexEF-oprN | 37 |
| YM44 | YM34 ΔmexXY | 37 |
| Plasmids | ||
| pEX18Tc | Broad-host-range gene replacement vector; sacB Tcr | 22 |
| pYM008 | pEX18Tc::ΔPA5471 | This study |
| pYM015 | pEX18Tc::ΔPA5471-PA5470 | This study |
| pYM021 | pEX18Tc::ΔmexZ | This study |
| pYM022 | pEX18Tc::ΔssrA | This study |
| pYM023 | pEX18Tc::ΔsmpB | This study |
| pUCP20T | Broad-host-range cloning vector; Mob+ Apr Cbr | 53 |
| pYM010 | pUCP20T::PA5471 | This study |
| pYM013 | pUCP20T::PA5471-PA5470 | This study |
| pYM017 | pUCP20T::mexZ | This study |
| pBC KS(+) | Phagemid cloning vector; Cmr | Stratagene |
| pTEM4 | pBR322::mexXY; Apr | 35 |
| pMMB190 | Broad-host-range, low-copy-number cloning vector; lacIq Apr Cbr | 34 |
| pYM004 | pMMB190::mexXY | This study |
a
Tcr, tetracycline resistance; Apr, ampicillin resistance; Cbr, carbenicillin resistance; Cmr, chloramphenicol resistance.