FIG. 5.

Inhibition of in vitro lipid II synthesis by gallidermin, epidermin, gallidermin mutant peptides, and nisin. Gallidermin and nisin were tested at molar ratios of 0.5:1 (white bars), 1:1 (gray bars), and 1.5:1 (black bars) with regard to the primary substrate C₅₅-P (10 nmol). Epidermin and gallidermin variants were tested only at a 1:1 (lantibiotic/substrate) molar ratio. The amount of lipid II synthesized by M. flavus membranes in an assay without addition of lantibiotics was taken as 100%. Inhibition of lipid II formation results from complexation of the peptides with the intermediate substrate lipid I. Mean values and standard deviations from three independent experiments are shown.