FIG. 7.
Inhibition of in vitro lipid II-Gly1 synthesis by gallidermin, epidermin, nisin, and teicoplanin (lanes and bars 2 to 5, respectively). (A) TLC of reaction mixtures of purified lipid II incubated with [U-14C]glycine in the presence of recombinant tRNA synthetase and purified tRNA with FemX in the absence (lane-1) or in the presence (lanes 2 to 5) of the peptides. (B) Ratio of [U-14C]glycine incorporated per lipid II as determined from the TLC presented in panel A. The results of one representative experiment are shown.