FIG. 4.

Cell contents in gentamicin as a function of its extracellular concentration and time. Electroporated (left panel), cells were subjected to electroporation in the presence of gentamicin at the concentrations indicated by the closed symbols at the top of the graphs and returned to drug-free medium for incubation at 37°C for the times indicated on the abscissa; incubated (right panel), cells were incubated at 37°C in the presence of gentamicin at the concentrations indicated by the open symbols at the top of the graphs for the times indicated on the abscissa. Values are means ± standard deviations (n = 3). (Insets) Correlation between the apparent cellular concentration (Cc) and the extracellular concentration (Ce) measured 1 h after electroporation for electroporated cells (left; slope, 6.3 ± 0.5) and after 24 h for incubated cells (right; slope, 0.76 ± 0.02).