TABLE 5.
Size- and chemistry-dependent ON inhibition of 6-HB formation by ELISA and FN-PAGE
| Chemistry | Size (bases) | 6-HB (ELISA) IC50 (μM, mean ± SD) | 6-HB inhibition (FN-PAGE)a |
|---|---|---|---|
| Randomer 1 | 6 | >100 | − |
| 10 | >100 | − | |
| 20 | >100 | ++ | |
| 30 | 9.80 ± 2.65 | ++ | |
| 40 | 8.37 ± 0.67 | ++ | |
| 50 | 0.18 ± 0.09 | +++ | |
| 60 | 0.39 ± 0.12 | +++ | |
| 80 | 0.83 ± 0.07 | +++ | |
| 120 | 0.23 ± 0.02 | +++ | |
| Randomer 2 | 6 | >100 | − |
| 10 | >100 | − | |
| 20 | 24.94 ± 4.13 | ++ | |
| 30 | 1.20 ± 0.13 | ++ | |
| 40 | 0.47 ± 0.001 | +++ | |
| 50 | 0.48 ± 0.07 | +++ | |
| Randomer 3 | 6 | >100 | − |
| 10 | >100 | − | |
| 20 | >100 | − | |
| 40 | >100 | − |
a
With 200 μM Randomer. −, no inhibition; ++, moderate inhibition; +++, >90% inhibition.