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Modified sandwich bioassay. On the right, three 20-μl spots of vancomycin-resistant E. faecalis R2526 are pipetted onto methicillin-resistant S. aureus (MRSA 494), with a resultant inhibition zone ± standard deviation of 8.07 ± 0.98 mm. The left side depicts the inhibitory effects of α-chymotrypsin and trypsin when added to the agar assay, with a resultant inhibition zone ± standard deviation of 2.23 ± 0.25 mm.
