TABLE 1.
Features of ORFs and discrete DNA segments in Tn2610
| Locationa (5′-3′) | DNA segment | ORFb
|
% Homology (DNA/aa) | Description of gene or gene product (accession no.) | ||
|---|---|---|---|---|---|---|
| No. | Name | Sizec | ||||
| 1-38 | IR | 38-bp IR in 1Ra of Tn2610 | ||||
| 34-3000 | S01 | tnpA | 988 | 99.7/99.2 | Transposase from Tn1721 (X61367) | |
| 3004-3564 | S02 | tnpR | 186 | 99.8/100 | Resolvase from Tn1721 (X61367) | |
| 3627-3755 | res | resIII (3627-3656), resII (3661-3703), resI (3717-3755) | ||||
| 3740-4324 | S03 | tnpM | 194 | 100/100 | TnpM on Tn21 (AF071413) | |
| 4091-5376 | 5′-CS | Partial 5′-CS of class 1 integron (AF261825) | ||||
| 4091-4114 | IRi | IRi of class 1 integron (AF261825) | ||||
| 4293-5306 | S04 | intI1 | 337 | 100/100 | Integrase of class 1 integron (AY214164) | |
| 5873-6079 | S05 | small ORF | 68 | 100/100 | Small ORF from pIP1527 (X03988) | |
| 6079-7338 | S06 | ereB | 419 | 100/100 | Erythromycin esterase type II (X03988) | |
| 7661-9145 | S07 | orf2 | 494 | 100/100 | Putative OrfA from SGI1 (AF261825) | |
| 9420-10073 | S08 | groEL/intI1 | 217 | 100/100 | GroEl/integrase fusion protein from SGI1 | |
| 9777-10209 | 5′-CS | Partial 5′-CS of class 1 integron (AF261825) | ||||
| 10154-10209 | attI | attI of class 1 integron (AF261825) | ||||
| 10279-11145 | S09 | pse-1 | 288 | 100/100 | PSE-1 β-lactamase from SGI (AF261825) | |
| 11275-12054 | S10 | aadA2 | 259 | 100/100 | Streptomycin resistance protein (AF164956) | |
| 12056-12114 | 59 bp | 59-bp element of class 1 integron (AF261825) | ||||
| 12114-14134 | 3′-CS | 3′-CS of class 1 integron (AF261825) | ||||
| 12218-12565 | S11 | qacEΔ1 | Quaternary ammonium compound and disinfectant resistance partial protein on SGI | |||
| 12559-13398 | S12 | sul1 | 279 | 100/100 | Sulfonamide resistance protein on SGI | |
| 13526-14026 | S13 | orf5 | 166 | 100/100 | Putative acetyltransferase in Tn21 (AF071413) | |
| 14050-14134 | S14 | orf6Δ | Hypothetical partial protein (AF261825) | |||
| 14135-14160 | IR | Terminal IR of IS1326 (AY123253.3) | ||||
| 14202-14987 | S15 | istB | 261 | 100/100 | Unknown function of IS1326 (AY123253.3) | |
| 14974-16497 | S16 | istA | 507 | 100/100 | Possible transposase of IS1326 (AY123253.3) | |
| 16579-16590 | IR | Terminal IR of IS1353 (AY123523) | ||||
| 16620-18164 | S17 | OrfAB | 516 | 100/100 | OrfAB from IS1353 (AY123523) | |
| 18180-18192 | IR | Terminal IR of IS1353 (AY123253.3) | ||||
| 18195-18280 | IR | Terminal IR of IS1326 (AY123253.3) | ||||
| 18215-19075 | S18 | tniBΔ1 | Truncated TniB from In2 (U42226) | |||
| 19078-19512 | S19 | tniA | Partial TniA of In2 (U42226) | |||
| 19513-19527 | IR | IR of IS26 (AY123523) | ||||
| 19575-20282 | S20 | tnpA | 235 | 100/100 | Transposase of IS26 (AY123523) | |
| 20321-20333 | IR | IR of IS26 (AY123523) | ||||
| 20334-20881 | S21 | tnpR | Truncated resolvase of Tn21 (AF071413) | |||
| 20884-23850 | S22 | tnpA | 988 | 100/100 | Transposase of Tn21 (AF071413) | |
| 23846-23883 | IR | 38-bp IR in IRb of Tn2610 | ||||
a
Nucleotide position in the sequence deposited under accession no. AB207867.
b
Named ORFs are based on those previously characterized.
c
Expressed as the number of amino acid residues.