FIG. 4.

Construction of the ΔrpoB2 mutant. A. Strategy for making an in-frame, unmarked deletion of rpoB2. See text for details. Δ indicates a deletion allele. The ScaI half-site generated after ligation of a 4.9-kb ScaI-SphI fragment containing rpoB2 to pUC19 digested with HincII and SphI is shown in parentheses. B. Southern hybridization analysis of a ΔrpoB2 mutant. NotI-digested total DNAs extracted from the wild-type strain (lane 1) and a ΔrpoB2 mutant (lane 2) were probed with a 0.6-kb EcoRI fragment containing nfa46450 (short black bar in panel A).