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CSF and MCF susceptibilities of S. cerevisiae cells hyperexpressing C. albicans drug efflux pumps in agar plate assays. Alleles were cloned from C. albicans ATCC 10261. Twofold serial dilutions of exponentially growing cells (5 μl) were spotted onto YPD agar medium containing CSF or MCF at the indicated concentrations and incubated at 30°C for 48 h as described in Materials and Methods. Representative results of an experiment carried out three times are shown.
