FIG. 3.

Disruption or overexpression of CgPDR1 altered the expression of CgCDR1 and PDH1. Northern hybridization analyses were used to determine the expression of CgPDR1, CgCDR1, and PDH1. Ten micrograms of total RNA was used for Northern blot analysis. The membrane was hybridized with the following probe: 2.3-kb BglII CgPDR1 DNA (top panel), 1.1-kb NotI-BamHI CgCDR1 DNA from pCRScript-CDR1 (14) (middle panel), or 3.6-kb PDH1 DNA from pClaI (25) (bottom panel). Sizes of putative transcripts are indicated in kilobases on the right. The rRNA stained with ethidium bromide was used as the loading control. Lanes: 1, strain NCCLS84, wild type; 2, strain CgB4, Cgpdr1 mutant; 3, strain CgB4/pCgACU, Cgpdr1 mutant carrying the vector pCgACU; 4, strain CgB4/pCgPDR1, Cgpdr1 mutant overexpressing CgPDR1. WT, wild type.