FIG. 1.

Analysis of synthetic human −2 RANTES toxicity in vitro. HeLa cells were exposed to increasing concentrations of −2 RANTES or N-9 (positive control) for 30 min (A), 2 h (B), 4 h (C), and 24 h (D). Microbicide concentrations up to 1 mg/ml of −2 RANTES and N-9 were evaluated at the indicated time points. Cellular viability was measured immediately following microbicide exposure by using an MTS cellular viability assay. The results are expressed relative to those for mock-exposed cells. The data in each graph represent the average of three experiments, in which each concentration was tested in triplicate. Error bars indicate the standard deviations of the calculated mean values.