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. 2003 Feb;185(3):788–800. doi: 10.1128/JB.185.3.788-800.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 5. — Binding specificity of RmpA2 to its target promoters. The ³²P-labeled DNA probes of P_orf3-15 (A), P_orf1-2 (B), and P_rmpA2 (C) were incubated with increasing amounts of His-RmpA2₂₁₂ as indicated. Lanes: 1, no protein; 2, 64 ng of protein; 3, 128 ng of protein; 4, 250 ng of protein; 5 to 8, 500 ng of protein. Specific inhibition of binding was investigated by adding the indicated amounts of unlabeled DNA fragment of a target promoter: lane 6, 1 ng; lane 7, 10 ng; lane 8, 100 ng.

FIG. 5. — Binding specificity of RmpA2 to its target promoters. The ³²P-labeled DNA probes of P_orf3-15 (A), P_orf1-2 (B), and P_rmpA2 (C) were incubated with increasing amounts of His-RmpA2₂₁₂ as indicated. Lanes: 1, no protein; 2, 64 ng of protein; 3, 128 ng of protein; 4, 250 ng of protein; 5 to 8, 500 ng of protein. Specific inhibition of binding was investigated by adding the indicated amounts of unlabeled DNA fragment of a target promoter: lane 6, 1 ng; lane 7, 10 ng; lane 8, 100 ng.