FIG. 9.

Response of RmpA2 target promoters. The luxAB fusions were cotransformed individually with the RmpA2 expression vector pET-RmpA2₂₁₂ into E. coli NovaBlue(DE3). Upon 1 mM IPTG induction, the luciferase activity of P_rmpA2::luxAB (black bars), P_orf3-15::luxAB (light gray bars), or P_orf1-2::luxAB (gray bars) was measured every hour and represented as the activation relative to that at time zero. The amount of His-RmpA2 synthesized in E. coli NovaBlue(DE3) after IPTG induction was determined by using a His tag-specific MAb followed by counting with a densitometer, and the result is shown on the right-hand axis (closed circles).