TABLE 2.
Primers used in this study
| Primer no. | Sequence | Enzyme cleaved | Complementary position |
|---|---|---|---|
| 020 | 5′-CTGTGTGATTAAGAATTCATA-3′ | 5′-EcoRI | −223 relative to the rmpA2 start codon |
| 021 | 5′-ATCCTCTAGAGTCGACGCGTT-3′ | 5′-XbaI | −1392 relative to the rmpA2 start codon |
| 024 | 5′-CATGCCATGGGAAAATATATTTAC-3′ | 5′-NcoI | +1 of the rmpA2 coding region |
| 025 | 5′-ATATCTCGAGTTATCTAGGTATTTGATG-3′ | 5′-XhoI | +636 of the rmpA2 coding region |
| 026 | 5′-ACGCGGATCCTAGCTCCACAGGTAAAGT-3′ | 5′-BamHI | −812 relative to the rmpA2 start codon |
| 027 | 5′-ACGCGGATCCGTCCAGTTAACTGCTTTA-3′ | 5′-BamHI | +96 relative to the rmpA2 start codon |
| 028 | 5′-ATATGGATCCCACTTAGTCCTGTGTCCA-3′ | 5′-BamHI | −390 relative to the rmpA2 start codon |
| 040 | 5′-ACTGGATCAGGCCTGGTAATCGCCATT-3′ | 5′-BamHI | −891 relative to the orf3 start codon |
| 041 | 5′-ACTGGATCCCGCTGTCGTATCTCAATG-3′ | 5′-BamHI | +60 relative to the orf3 start codon |
| 049 | 5′-GCCGAGCTCTGGACATAGACAGC-3′ | 5′-SacI | +639 relative to the rmpA2 stop codon |
| 050 | 5′-CCGGAATTCCTAAAGGGTGTGATTATG-3′ | 5′-EcoRI | +295 relative to the rmpA2 start codon |
| 051 | 5′-ATATCTCGAGTTAAATTTCCTTGCATGTTGAC-3′ | 5′-HindIII | +333 of the rmpA2 coding region |
| 052 | 5′-CCCAAGCTTCAGTGGTGGTGGTGGTGGTGGGTATTTGATGTGC ACCA-3′ | 5′-HindIII | +636 of the rmpA2 coding region |
| 053 | 5′-TGCTCTAGAAGCGACTCATCGATCAG-3′ | 5′-XbaI | +999 relative to the lon stop codon |
| 054 | 5′-CCCAAGCTTGCGCTGCAGAACGCC-3′ | 5-HindIII | +61 relative to the lon stop codon |
| 055 | 5′-CCCAAGCTTCACGCGCTCCAGGCCATA-3′ | 5-HindIII | +993 relative to the lon start codon |
| 056 | 5′-AGAGAGCTCTATGAATCCTGAGCG-3′ | 5′-SacI | +10 relative to the lon start codon |
| 061 | 5′-AGAGAGCTCTGCAGCTGCTCATCAACA-3′ | 5′-SacI | −983 relative to the rcsB start codon |
| 062 | 5′-CCCAAGCTTGCGCATCCTTTTCGCGA-3′ | 5′-HindIII | −10 relative to the rcsB start codon |
| 063 | 5′-CCCAAGCTTATTCCCGCCCTTTACGCA-3′ | 5-HindIII | +47 relative to the rcsB stop codon |
| 064 | 5′-TGCTCTAGAGGGGATCCCGGCGAAA-3′ | 5′-XbaI | +1018 relative to the rcsB stop codon |
| 074 | 5′-ACTGGATCCACGATCATGGATAAGAT-3′ | 5′-BamHI | −723 relative to the orf1 start codon |
| 075 | 5′-ACTGGATCCTGCGACCGGAATAACC-3′ | 5′-BamHI | +42 relative to the orf1 start codon |
| 097 | 5′-CCCAAGCTTCTAAGCGTAGTCTGGGACGTCGTATGGGTAGGTATTT GATGTGCACCAA-3′ | 5′-HindIII | +636 of the rmpA2 coding region |