TABLE 3.
Effects of RmpA2 on CPS synthesis in K. pneumoniae CG43 strains with different genetic backgrounds
| Strains | CPS synthesized when grown as indicatedd
|
Mucoid phenotypec | |||||
|---|---|---|---|---|---|---|---|
| LB incubation
|
Minimal medium (0.4% glucose)
|
Minimal medium (1% glycerol)
|
|||||
| Mean quantity ± SDa | Foldb | Mean quantity ± SD | Fold | Mean quantity ± SD | Fold | ||
| CG43S3 | 15.4 ± 0.7 | 110.4 ± 3.9 | 51.6 ± 6.3 | + | |||
| R2035 | 13.5 ± 0.5 | 79.1 ± 3.6 | 48.4 ± 5.1 | − | |||
| B2202 | 12.9 ± 0.8 | 72.2 ± 2.9 | 33.3 ± 2.8 | − | |||
| L2117 | 97.4 ± 2.8 | 600.6 ± 32.4 | 232.6 ± 9.7 | +++ | |||
| RB01 | 10.4 ± 1.2 | 62.3 ± 4.7 | 27.3 ± 5.6 | − | |||
| RL01 | 10.9 ± 1.4 | 60.7 ± 5.3 | 35.1 ± 6.2 | − | |||
| S3[pYC084] | 29.2 ± 2.5 | 1.89 | 166.1 ± 6.2 | 1.50 | 65.3 ± 6.4 | 1.27 | ++ |
| R2035[pYC084] | 30.9 ± 1.8 | 2.29 | 235.3 ± 13.4 | 2.97 | 87.4 ± 8.9 | 1.81 | ++ |
| B2202[pYC084] | 31.4 ± 2.7 | 2.43 | 158.5 ± 8.4 | 2.20 | 61.2 ± 7.7 | 1.84 | ++ |
| L2117[pYC084] | 111.8 ± 6.7 | 1.15 | 814.3 ± 31.5 | 1.36 | 417.3 ± 9.6 | 1.79 | +++ |
| RB01[pYC084] | 30.3 ± 3.2 | 2.91 | 188.5 ± 5.8 | 3.03 | 72.0 ± 3.5 | 2.64 | + |
| RL01[pYC084] | 26.1 ± 2.9 | 2.39 | 268.2 ± 7.5 | 4.42 | 67.8 ± 6.4 | 1.93 | ++ |
a
Values are the averages of triplicate samples and are given as micrograms of uronic acid per 109 CFU.
b
Compared with each parental strain without pYC084.
c
Assessed by string formation test after 48 h of growth on M9-glucose medium. Symbols: −, negative; +, positive; ++, strong; +++, very strong.
d
The production of polysaccharides on the cell surface of each strain was ascertained as K2 CPS by the Quellung test.