TABLE 5.
Effect of RmpA2 on expression of luxAB transcriptional fusions in E. coli NovaBlue(DE3)
| Strain | Mean LuxAB activity ± SD (RLU/OD600)a
|
||
|---|---|---|---|
| Porf3-15::luxAB | Porf1-2::luxAB | PrmpA2::luxAB | |
| NovaBlue(DE3) | 215 ± 13 | 5 ± 0.74 | 12,638 ± 2,168 |
| NovaBlue(DE3)[pET-RmpN111] | 328 ± 52 | 9 ± 1 | 11,237 ± 1,045 |
| NovaBlue(DE3)[pET-Rmp212] | 1,236 ± 83 | 104 ± 2 | 1,813 ± 112 |
a
Luciferase activity was measured after 8 h of induction with 1 mM IPTG at 25°C. Values are the averages of triplicate samples. RLU, relative light units.