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PCR amplification of the rrnB region from strains used in the relative fitness assay. Amplification of the rrnB operon from BSV1 sacB mutant strains, which contain the sacB-neo cassette, results in a product of approximately 10 kbp. Amplification of the recombinant or wild-type rrnB operon results in a product of approximately 6.6 kbp.
