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Presence of the (phosphorylated) Wzc protein in defined A. lwoffii RAG-1 mutants. Lysates of the RAG-1 mutants were immunoblotted using antiphosphotyrosine antibodies (A) and anti-Wzc (B) as described in Materials and Methods. Lane 1, wild-type RAG-1; lane 2, LN501; lane 3, LN502; lane 4, LN503; lane 5, LN504.
