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Size distribution of the polysaccharides in the supernatant of defined mutants of A. lwoffii RAG-1 compared to the wild type. Samples of the supernatant were run on an SDS-polyacrylamide gel and stained with Alcian blue as described in Materials and Methods. Lane M, broad-range protein marker. Lane 1, wild-type RAG-1; lane 2, LN501; lane 3, LN502; lane 4, LN503; lane 5, LN504.
