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. 2003 Feb;185(3):870–878. doi: 10.1128/JB.185.3.870-878.2003

TABLE 2.

Plasmids used in this study

Plasmid	Relevant features^a	Source or reference(s)
pBR322	Ap^r Tc^r; cloning vector	5
pUC19	Ap^r; cloning vector	59
pUC18Ery	Ap^r Em^r; derivative of pUC18 carrying an ery gene	50
pUC19Ery	Ap^r Em^r; derivative of pUC19 carrying an ery gene	32
pNZ8020	Cm^r; inducible expression vector carrying the nisA promoter	14
pNZ8400	Ap^r; 2.4-kbp SstI fragment of Lactococcus lactis cloned in pUC19	This study^b
pNZ8401	Ap^r; pNZ8400 derivative with a ScaI/StuI 380-bp deletion in the galK reading frame	This study^b
pNZ8402	Ap^r Em^r; XbaI/PstI fragment of pUC19Ery containing the ery gene and cloned in pNZ8401	This study^b
pNZ8410	Ap^r; 7.3-kbp SalI/EcoRI fragment of Lactococcus lactis cloned in pBR322	This study^b
pNZ8421	Ap^r; 1.8-kbp EcoRI fragment of pNZ8410 containing galE cloned in pBR322, orientation 1	This study^b
pNZ8422	Ap^r; same as pNZ8421, orientation 2	This study^b
pNZ8442	Ap^r; 2.5-kbp SalI/NheI fragment of pNZ8410 containing galP cloned in pUC18	This study^b
pNZ8451	Ap^r Em^r; 1.1-kbp internal HpaII fragment of galP cloned in pUC18Ery, orientation 1	This study^b
pNZ8452	Ap^r Em^r; same as pNZ8452, orientation 2	This study^b
pNZ8460	Ap^r Em^r; internal 0.8-kbp fragment of galE cloned in pUC18Ery	This study^b
pNZ8560	Cm^r; 1.8-kbp EcoRI fragment of pNZ8410 containing galE cloned in pNZ8020	This study^b

^a

Em^r, Ap^r, Cm^r, and Tc^r, resistance to erythromycin, ampicillin, chloramphenicol, and tetracycline, respectively.

^b

See Materials and Methods and Fig. 1.