FIG. 1.

ScaA sequencing strategy and domain architecture. The sequence of ScaA was completed by PCR walking, by sequencing extended products produced with ScaAcoh5r (∼0.8 kb) and then with 7r (∼0.9 kb) (Table 1; also see Materials and Methods). Additional primers and amplifications were used to obtain sequences for both strands and to confirm the relationship of the ScaA N terminus with unique ScaA dockerin, and ScaB sequences and their positions are also indicated. Domains within the ScaA protein are shown as follows: X, N terminal domain of unknown function; 1, 2, and 3, cohesin domains; Doc, dockerin. Solid boxes indicates threonine-rich linkers and striped boxes indicate N-terminal signal peptide.