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. 2003 Feb;185(3):703–713. doi: 10.1128/JB.185.3.703-713.2003

FIG. 2.

FIG. 2.

Detection of native ScaA with antibodies raised against purified, recombinant ScaA-Coh2. Proteins from supernatants of cellobiose-grown R. flavefaciens 17 cultures were separated by SDS-PAGE and analyzed by Coomassie blue staining (lane 1) or glycostaining (lane 2). A duplicate sample was transferred to PVDF membranes and probed with anti-ScaA-Coh2 antibodies (lane 3). Numbers at left are molecular masses in kilodaltons.