FIG. 4.

Recognition of a common 130-kDa protein band by different cohesin domains from ScaB. Total R. flavefaciens 17 supernatant proteins (approximately 7 μg) from a cellulose-grown culture were loaded onto successive lanes of an SDS-PAGE gel, separated, and subjected to Western blotting. Membrane strips containing the electrophoretically transferred material were then incubated with each of the designated cohesin probes (standardized by His tag detection, corresponding to approximately 10 to 50 μg of protein). Binding was detected by chemiluminescence using peroxidase-conjugated anti-His tag antibody. Lanes are marked 1 through 7 according to the cohesin probe used. Lane Rf, profile of Coomassie-stained R. flavefaciens supernatant proteins. Numbers at left are molecular masses in kilodaltons.