FIG. 9.

Phylogenetic relationship of R. flavefaciens dockerin domains. Dockerins from the indicated R. flavefaciens enzymes (designated Rumfl-EndA, etc.) are shown as filled circles. Scaffoldin-borne dockerins from R. flavefaciens (Rumfl-ScaA), C. thermocellum (Clotm-CipA), A. cellulolyticus (Acece-CipV), and B. cellulosolvens (Bacce-CipBc) are shown as squares. Other dockerin-borne enzymes are from R. albus (Rumal-EgV, etc.), Ruminococcus sp. (Rumsp-Xyn1), and a selection of enzymes from C. thermocellum and mesophilic clostridia (C. cellulolyticum, C. cellulovorans, and C. josui). For a precise list of the proteins and their accession numbers, consult Materials and Methods. The scale bar indicates the percentage (0.1) of amino acid substitutions.