TABLE 1.

Oligonucleotide primers used in this study^a

Name	Sequence	Domain(s) amplifiedb
ScaAcoh1r2	ATGTAGGAACGAGCTTTGTGC	ScaA dockerin (rev)
ScaAcoh5r	ATCAGCATTTGATGTCTGGC	ScaA cohesins (rev)
ScaAcoh7f	CAGTCGGCACTGTAACACC	ScaA X domain (fwd)
ScaAcoh7r	GCGAGGTTGTACTTGGAC	ScaA cohesins (rev)
ScaAcoh8f	GCTCCTTCAACTGTAGGC	ScaA X domain (fwd)
ScaAcoh8r	GCTGAGAAGTAAGGCTGGTC	ScaA X domain (rev)
ScaAcoh9f	GTCCAAGTACAACCTCGC	ScaA X domain (fwd)
AUnk-NcoI	TATACCATGGCTCCTTCAACTGTAGGCGCT	ScaA N terminal (fwd)
AUnk-XhoI	AAATCTCGAGTGTACCGGGTGTTGAACCGT	ScaA N terminal (rev)
Bcoh1-NcoI	ATATCCATGGCTGCCGGTGGTTTA	ScaB cohesin 1 (fwd)
Bcoh1-XhoI	CGGTCTCGAGATCCTTCTTAACAAT	ScaB cohesin 1 (rev)
Bcoh2-NcoI	AAAACCATGGTCAACAACACAGGTAAG	ScaB cohesin 2 (fwd)
Bcoh2-XhoI	CTCACTCGAGATCATTCTTGACTGTGAT	ScaB cohesin 2 (rev)
Bcoh3-NcoI	CAATCCATGGTACAGACGGTAGGTAAA	ScaB cohesin 3 (fwd)
Bcoh3-XhoI	AAAACTCGAGTACAGGCTTGCCGAC	ScaB cohesin 3 (rev)
Bcoh6-NcoI	AAAACCATGGCTCCTCAGGGCGGTATC	ScaB cohesin 6 (fwd)
Bcoh6-XhoI	ATCACTCGAGAATAACGGGCTTCTTTAC	ScaB cohesin 6 (rev)
Bcoh7-NcoI	AATTCCATGGTAGTTCCCAAGGATTCT	ScaB cohesin 7 (fwd)
Bcoh7-XhoI	ATATCTCGAGAGGAGCGGGCTTTTCAAA	ScaB cohesin 7 (rev)
EndB-NcoI	AATTCCATGGCGCCCGTCAACGGTCTG	EndB full length (fwd)
EndB-XhoI	CACGCTCGAGTTCGGGAAGCTTGTCTAT	EndB full length (rev)
XynE-NcoI	CTGACCATGGTTACAAATCCTACTATCAAT	XynE dockerin + family 1 carbohydrate esterase (fwd)
XynE-XhoI	AGTGCTCGAGCTTGAAAGCATATCTGAAGA	XynE dockerin + family 1 carbohydrate esterase (rev)
XynB-EcoRI	AAGGAATTCACCACACAGCCTTCGAGC	XynB family 3 carbohydrate esterase + dockerin (fwd)
XynB-XhoI	AACCGCTCGAGCTTGCAACTTTACCGATGAGG	XynB family 3 carbohydrate esterase + dockerin (rev)
CesA-EcoRI	AAGGAATTCAAGCACTTTGTTGTCGG	CesA family 3 carbohydrate esterase + dockerin (fwd)
CesA-XhoI	AACCGCTCGAGCAGGGAAGTTGTAGC	CesA family 3 carbohydrate esterase + dockerin (rev)

^a Residues shown in italics were added to the 5′ ends to generate restriction enzyme cleavage sites (underlined).

^bAbreviations: fwd, forward; rev, reverse.