TABLE 2.
Predicted specificity residues of dockerins derived from the indicated proteins
| Enzyme or dockerin | Protein | Residues in duplicated segmenta
|
|
|---|---|---|---|
| 1st | 2nd | ||
| Ruminococcal enzyme from: | |||
| R. flavefaciens | EndA | LA | GD |
| EndB | LA | ND | |
| XynB | LA | AD | |
| XynD | LS | GD | |
| CesA | SF | VA | |
| XynE | SL | VA | |
| Ruminococcus sp. strain | Xyn1 | SF | VA |
| R. albus | EgV | VT | VS |
| EgVI | MA | ND | |
| EgVII | DT | MA | |
| C. thermocellum enzymes | Consensusb | ST | ST |
| Mesophilic clostridial enzymes | Consensusb | A(L/I) | A(I/L) |
| Scaffoldin dockerins from: | |||
| R. flavefaciens | ScaA | VA | AV |
| C. thermocellum | CipA | LL | MQ |
| A. cellulolyticus | CipV | LE | LE |
| B. cellulosolvens | CipBc | SD | SD |
a
The four residues indicated represent positions 10 and 11 of the calcium-binding motifs in the first and second duplicated segments, respectively (35).
b
The consensus residues represent the dominant amino acid(s) that appears in the designated position from the indicated group of cellulosomal enzymes.