FIG. 2.
Pbp4* accumulates in ftsH knockout strains. Immunoblot analysis detects increased amounts of PBP4* in ftsH null mutants. The different B. subtilis strains indicated were grown to the mid-exponential-growth phase in LB at 37°C. Proteins present in cell lysates were separated by SDS-PAGE, blotted, and developed with polyclonal antibodies against PBP4*, FtsH, or (as an internal control) HtpG.