FIG. 3.

Accumulation of Pbp4* in ftsH knockout strains is due to enhanced transcription of pbpE. (A) Schematic drawing showing fusion of the pbpE gene to the xylose-regulatable promoter P_xylA. Comparable amounts of PBP4* are present in the presence and absence of ftsH. (B) Proteins from cells grown as described above were separated by SDS-PAGE and probed with three different antibodies as indicated. +, presence of xylose; −, absence of xylose. (C) Northern blot analysis reveals increased amounts of pbpE transcript in the ftsH knockout mutant. Total RNAs were prepared from B. subtilis 1012 and its ftsH::erm derivative at the OD₅₇₈ indicated and were hybridized with digoxigenin-labeled pbpE antisense RNA.