TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| XL-1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqlacZM15 Tn10] | Stratagene |
| DH10B | mcrA Δ(mrr hsdRMS mcrBC) φ80d lacZM15 ΔlacX74 deoR recA1 araD139 Δ(ara leu)7697 galU galK rpsL endA1 nupG | Bethesda Research Laboratories, Inc. |
| Bacillus subtilis | ||
| 1012 | leuA8 metB5 trpC2 hsrM1 | 23 |
| WW01 | ftsH::erm derivative of 1012 | 36 |
| ED04 | ftsH::tet in 1012 | 9 |
| MP01 | ftsH::cat in 1012 | 9 |
| sigW | sigW::neo allele in 1012 | 37 |
| SWV119 | abrB::tet | 30 |
| ΔpbpE | ΔpbpE::erm allele isolated from PS1805 and transformed into 1012 | 21 |
| BFS233 | pMUTIN inserted into yuaF; promoter of yuaF fused to lacZ | 27 |
| MSW01 | pX2-ftsH | This work |
| SZ01 | ΔpbpE::erm ftsH::cat | This work |
| SZ06 | ΔpbpE::erm amyE::PxylA-pbpE ftsH+ in 1012 | This work |
| SZ08 | ΔsigW::neo pX2-ftsH | This work |
| SZ09 | ΔpbpE::erm amyE::PxylA-pbpE ftsH::tet in 1012 | This work |
| TW51 | amyE::PyuaF-lacZ; derived from pPyuaF-bgaB, with bgaB replaced by lacZ | This work; PyuaF-bgaB from reference 37 |
| TW52 | amyE::PyuaF-lacZ ftsH::cat | This work |
| Plasmids | ||
| pQE30 | 3.5-kb vector allowing overproduction of proteins with six N-terminal histidine residues | 32 |
| pX | Carries xylose-regulatable expression cassette for insertion at the amyE locus | 14 |
| pX2 | Carries xylose-regulatable expression cassette to be fused in front of the gene of interest | 18 |