FIG. 2.

Multiple-sequence alignment of LlpA (LlpA_Pseput), the precursor protein of ASAI, a mannose-specific lectin found in bulbs of garlic (A. sativum) (ASAI_Allsat), and hypothetical protein XAC0868 from X. axonopodis pv. citri (XAC0868_Xanaxo). Gaps are indicated by dots. Predicted MMBL domains are enclosed in boxes. Amino acids involved in mannose binding in ASAI are indicated by solid circles when they are present in LlpA or by open circles when they are not present in LlpA. The labeled tryptophan residues (asterisks) are part of a stabilizing hydrophobic core structure and are conserved in nearly all homologous proteins. Cysteine residues typical of plant MMBL domains are also identified (inverted open triangles). The signal peptide of ASAI is double underlined. A SignalP search revealed the presence of a putative 21-amino-acid signal sequence (underlined) for XAC0868. The linker peptide of ASAI which is cleaved off during final processing is underlined with a dashed line. The N-terminal amino acid sequence of LlpA determined by sequencing is also underlined.