TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristic(s) | Source or reference |
|---|---|---|
| E. coli strains | ||
| TOP10F′ | F′[lacIq Tn10(Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacZ74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| GM2163 | F−ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam-13::Tn9(Camr) xylA5 mtl-1 thi-1 mcrB1 hsdR2 | New England Biolabs |
| Pseudomonas sp. strains | ||
| BW11M1 | Strain isolated from banana rhizosphere | 63 |
| CMPG2065 | BW11M1 TnMod mutant; Kmr | This study |
| P. agarici LMG 2112 | Type strain | BCCMc |
| P. aeruginosa PAO1 | 56 | |
| P. aureofaciens LMG 1245a | Type strain | BCCM |
| P. cichorii LMG 2162 | Type strain | BCCM |
| P. chlororaphis LMG 5004 | Type strain | BCCM |
| P. fuscovaginae HPB 2646 | H. Maraitéd | |
| P. fluorescens strains | ||
| 2-79 | Strain isolated from wheat rhizosphere | 65 |
| F113 | Strain isolated from sugarbeet rhizosphere | 52 |
| OE 28.3 | Strain isolated from wheat rhizosphere | 13 |
| SBW25 | Strain isolated from sugarbeet leaves | 47 |
| WCS365 | Strain isolated from potato rhizosphere | 20 |
| P. marginalis LMG 2210b | Pathovar reference strain | BCCM |
| P. mendocina LMG 1223 | Type strain | BCCM |
| P. putida strains | ||
| GR12-2R3 | Rifampin-resistant derivative of strain GR12-2 isolated from grass rhizosphere | 36 |
| LMG 2257 | Type strain | BCCM |
| KT2440 | pWW0-cured, restriction-deficient derivative of P. putida mt-2 | 18 |
| P. resinovorans LMG 2274 | Type strain | BCCM |
| P. stutzeri LMG 11199 | Type strain | BCCM |
| P. syringae pv. glycinea LMG 5066 | Pathovar reference strain | BCCM |
| P. syringae pv. syringae LMG 1247 | Type strain | BCCM |
| P. syringae pv. tabaci LMG 5192 | BCCM | |
| P. syringae pv. tomato DC3000 | 66 | |
| P. tolaasii LMG 2342 | Type strain | BCCM |
| P. viridiflava LMG 2352 | Type strain | BCCM |
| Plasmids | ||
| pUC18/pUC19 | ColE1 replicon, lacZ, cloning vector; Apr | 69 |
| pCR2.1 | ColE1 replicon, f1 origin, lacZ; Apr Kmr | Invitrogen |
| pWTT2081 | E. coli-Pseudomonas shuttle vector based on pACYC184 and pVS1 replicons; Tcr | 62 |
| pPDM-1 | Plasmid deletion vector; Apr Kmr | Epicentre |
| pTnMod-OKm′ | Plasposon vector with pMB1 replicon between inverted repeats; Kmr | 14 |
| pCMPG6010 | pUC18 containing 10.9-kb genomic KpnI fragment with llpA from BW11M1 | This study |
| pCMPG6012 | pUC18 with 989-bp PCR-amplified fragment containing llpA gene cloned in KpnI/BamHI | This study |
| pCMPG6015 | pUC19 with 989-bp PCR-amplified fragment containing llpA gene cloned in KpnI/BamHI | This study |
| pCMPG6018 | pWTT2081 with insert from pCMPG6012, blunted and recloned in SmaI/BamHI | This study |
| pCMPG6020 | pPDM-1 with KpnI insert from pCMPG6010 | This study |
a
Now classified as P. chlororaphis.
b
P. marginalis pv. marginalis.
c
BCCM, Belgian Coordinated Collections of Microorganisms (http://www.belspo.be/bccm/index.htm).
d
H. Maraité, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.