FIG. 5.

Substrate specificity of recombinant PF0272 (4-α-glucanotransferase). Starch hydrolysis (A) and 4-α-glucanotransferase (B) activities were determined by using thin-layer chromatograms after recombinant PF0272 (20 μg) was incubated at 90°C in 300 μl of 100 mM MOPS (pH 7.0) for the indicated times with the following substrates. (A) Each reaction contained 1% (wt/vol) soluble starch with the following additions: lane 1, none; lane 2, 20 mM glucose; lane 3, 20 mM maltose; lane 4, 20 mM trehalose; lane 5, 20 mM cellobiose; and lane 6, 20 mM sucrose. (B) Lanes: 1, 20 mM maltose (G2); 2, 20 mM maltotriose (G3); 3, 10 mM maltotetraose (G4); 4, 10 mM maltopentaose (G5); 5, 10 mM maltohexaose and 10 mM maltoheptaose (G7); and 6, 1.0% (wt/vol) starch (ST). A total of 2 μl was added to each lane. Lane S contains the standard sugars.