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Substrate specificity of native 4-α-glucanotransferase. Thin-layer chromatograms of cytoplasmic fraction (A) and, fractions (B and C) exhibiting 4-α-glucanotransferase activity after two chromatography steps (DEAE-Sepharose and hydroxyapatite, see Materials and Methods). The fractions were incubated with 20 mM maltose (A and B) or 20 mM maltotriose (C) in 100 mM MOPS (pH 7.0) at 90°C for the indicated times. Lane S contains standard sugars.
