FIG. 7.

Substrate specificity of recombinant PF 1535 (maltodextrin phosphorylase). Thin-layer chromatograms are shown after recombinant PF1535 (20 μg) was incubated in 100 mM potassium phosphate buffer (pH 7.0) with 20 mM glucose (G1), 20 mM maltose (G2), 20 mM maltotriose (G3), 10 mM maltotetraose (G4), 10 mM maltopentaose (G5), 10 mM maltohexaose and 10 mM maltoheptaose (G7), and 1.0% starch (St) for the indicated periods. Glucose-1-phosphate (G1P) was also applied as a standard and its point of migration is indicated by the arrow.