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. 2006 Mar;188(6):2063–2072. doi: 10.1128/JB.188.6.2063-2072.2006

FIG. 4.

FIG. 4.

CHEF gel and Southern hybridization analysis of transconjugants obtained from filter mating experiments employing mutant P101D12 as the donor and OG1RF as the recipient. (A) CHEF gel of I-ceuI-restricted DNA from three primary transconjugants, POTC-1, POTC-2, and POTC-3 (lanes 1, 2, and 3, respectively); donor strain P101D12 (lane 4); and recipient strain OG1RF (lane 5). (B and C) Also shown are Southern hybridization analyses of I-ceuI-restricted DNA from the transconjugants (lanes 1 to 3), donor strain P101D12 (lane 4), and recipient strain OG1RF (lane 5) using a 23S rRNA gene probe (B) and a Tn917-specific probe (C). Bacteriophage lambda concatemers were used as molecular size markers (lane M; New England Biolabs, Beverly, MA).